High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.
Identifieur interne : 000482 ( Main/Exploration ); précédent : 000481; suivant : 000483High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.
Auteurs : Émilie D. Tremblay [Canada] ; Troy Kimoto [Canada] ; Jean A. Bérubé [Canada] ; Guillaume J. Bilodeau [Canada]Source :
- Journal of fungi (Basel, Switzerland) [ 2309-608X ] ; 2019.
Abstract
Studying the means of dispersal of plant pathogens is crucial to better understand the dynamic interactions involved in plant infections. On one hand, entomologists rely mostly on both traditional molecular methods and morphological characteristics, to identify pests. On the other hand, high-throughput sequencing (HTS) is becoming the go-to avenue for scientists studying phytopathogens. These organisms sometimes infect plants, together with insects. Considering the growing number of exotic insect introductions in Canada, forest pest-management efforts would benefit from the development of a high-throughput strategy to investigate the phytopathogenic fungal and oomycete species interacting with wood-boring insects. We recycled formerly discarded preservative fluids from the Canadian Food Inspection Agency annual survey using insect traps and analysed more than one hundred samples originating from across Canada. Using the Ion Torrent Personal Genome Machine (PGM) HTS technology and fusion primers, we performed metabarcoding to screen unwanted fungi and oomycetes species, including Phytophthora spp. Community profiling was conducted on the four different wood-boring, insect-attracting semiochemicals; although the preservative (contained ethanol) also attracted other insects. Phytopathogenic fungi (e.g., Leptographium spp. and Merialaricis in the pine sawyer semiochemical) and oomycetes (mainly Peronospora spp. and Pythium aff. hypogynum in the General Longhorn semiochemical), solely associated with one of the four types of semiochemicals, were detected. This project demonstrated that the insect traps' semiochemical microbiome represents a new and powerful matrix for screening phytopathogens. Compared to traditional diagnostic techniques, the fluids allowed for a faster and higher throughput assessment of the biodiversity contained within. Additionally, minimal modifications to this approach would allow it to be used in other phytopathology fields.
DOI: 10.3390/jof5010015
PubMed: 30759800
PubMed Central: PMC6463110
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.</title><author><name sortKey="Tremblay, Emilie D" sort="Tremblay, Emilie D" uniqKey="Tremblay E" first="Émilie D" last="Tremblay">Émilie D. Tremblay</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Emilie.Tremblay@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9</wicri:regionArea><wicri:noRegion>K2H 8P9</wicri:noRegion></affiliation></author><author><name sortKey="Kimoto, Troy" sort="Kimoto, Troy" uniqKey="Kimoto T" first="Troy" last="Kimoto">Troy Kimoto</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 4321 Still Creek Dr, Burnaby, BC, V5C 6S7, Canada. Troy.Kimoto@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 4321 Still Creek Dr, Burnaby, BC, V5C 6S7</wicri:regionArea><wicri:noRegion>V5C 6S7</wicri:noRegion></affiliation></author><author><name sortKey="Berube, Jean A" sort="Berube, Jean A" uniqKey="Berube J" first="Jean A" last="Bérubé">Jean A. Bérubé</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Canada, Laurentian Forestry Centre, 1055 Du P.E.P.S. Street, P.O. Box 10380 Québec, QC, G1V 4C7, Canada. Jean.Berube@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Natural Resources Canada, Laurentian Forestry Centre, 1055 Du P.E.P.S. Street, P.O. Box 10380 Québec, QC, G1V 4C7</wicri:regionArea><wicri:noRegion>G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Bilodeau, Guillaume J" sort="Bilodeau, Guillaume J" uniqKey="Bilodeau G" first="Guillaume J" last="Bilodeau">Guillaume J. Bilodeau</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Guillaume.Bilodeau@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9</wicri:regionArea><wicri:noRegion>K2H 8P9</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30759800</idno><idno type="pmid">30759800</idno><idno type="doi">10.3390/jof5010015</idno><idno type="pmc">PMC6463110</idno><idno type="wicri:Area/Main/Corpus">000547</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000547</idno><idno type="wicri:Area/Main/Curation">000547</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000547</idno><idno type="wicri:Area/Main/Exploration">000547</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.</title><author><name sortKey="Tremblay, Emilie D" sort="Tremblay, Emilie D" uniqKey="Tremblay E" first="Émilie D" last="Tremblay">Émilie D. Tremblay</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Emilie.Tremblay@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9</wicri:regionArea><wicri:noRegion>K2H 8P9</wicri:noRegion></affiliation></author><author><name sortKey="Kimoto, Troy" sort="Kimoto, Troy" uniqKey="Kimoto T" first="Troy" last="Kimoto">Troy Kimoto</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 4321 Still Creek Dr, Burnaby, BC, V5C 6S7, Canada. Troy.Kimoto@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 4321 Still Creek Dr, Burnaby, BC, V5C 6S7</wicri:regionArea><wicri:noRegion>V5C 6S7</wicri:noRegion></affiliation></author><author><name sortKey="Berube, Jean A" sort="Berube, Jean A" uniqKey="Berube J" first="Jean A" last="Bérubé">Jean A. Bérubé</name><affiliation wicri:level="1"><nlm:affiliation>Natural Resources Canada, Laurentian Forestry Centre, 1055 Du P.E.P.S. Street, P.O. Box 10380 Québec, QC, G1V 4C7, Canada. Jean.Berube@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Natural Resources Canada, Laurentian Forestry Centre, 1055 Du P.E.P.S. Street, P.O. Box 10380 Québec, QC, G1V 4C7</wicri:regionArea><wicri:noRegion>G1V 4C7</wicri:noRegion></affiliation></author><author><name sortKey="Bilodeau, Guillaume J" sort="Bilodeau, Guillaume J" uniqKey="Bilodeau G" first="Guillaume J" last="Bilodeau">Guillaume J. Bilodeau</name><affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Guillaume.Bilodeau@canada.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9</wicri:regionArea><wicri:noRegion>K2H 8P9</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of fungi (Basel, Switzerland)</title><idno type="eISSN">2309-608X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Studying the means of dispersal of plant pathogens is crucial to better understand the dynamic interactions involved in plant infections. On one hand, entomologists rely mostly on both traditional molecular methods and morphological characteristics, to identify pests. On the other hand, high-throughput sequencing (HTS) is becoming the go-to avenue for scientists studying phytopathogens. These organisms sometimes infect plants, together with insects. Considering the growing number of exotic insect introductions in Canada, forest pest-management efforts would benefit from the development of a high-throughput strategy to investigate the phytopathogenic fungal and oomycete species interacting with wood-boring insects. We recycled formerly discarded preservative fluids from the Canadian Food Inspection Agency annual survey using insect traps and analysed more than one hundred samples originating from across Canada. Using the Ion Torrent Personal Genome Machine (PGM) HTS technology and fusion primers, we performed metabarcoding to screen unwanted fungi and oomycetes species, including <i>Phytophthora</i> spp. Community profiling was conducted on the four different wood-boring, insect-attracting semiochemicals; although the preservative (contained ethanol) also attracted other insects. Phytopathogenic fungi (e.g., <i>Leptographium</i> spp<i>.</i> and <i>Meria</i><i>laricis</i> in the pine sawyer semiochemical) and oomycetes (mainly <i>Peronospora</i> spp. and <i>Pythium</i> aff. <i>hypogynum</i> in the General Longhorn semiochemical), solely associated with one of the four types of semiochemicals, were detected. This project demonstrated that the insect traps' semiochemical microbiome represents a new and powerful matrix for screening phytopathogens. Compared to traditional diagnostic techniques, the fluids allowed for a faster and higher throughput assessment of the biodiversity contained within. Additionally, minimal modifications to this approach would allow it to be used in other phytopathology fields.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30759800</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2309-608X</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Feb</Month><Day>12</Day></PubDate></JournalIssue><Title>Journal of fungi (Basel, Switzerland)</Title><ISOAbbreviation>J Fungi (Basel)</ISOAbbreviation></Journal><ArticleTitle>High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E15</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/jof5010015</ELocationID><Abstract><AbstractText>Studying the means of dispersal of plant pathogens is crucial to better understand the dynamic interactions involved in plant infections. On one hand, entomologists rely mostly on both traditional molecular methods and morphological characteristics, to identify pests. On the other hand, high-throughput sequencing (HTS) is becoming the go-to avenue for scientists studying phytopathogens. These organisms sometimes infect plants, together with insects. Considering the growing number of exotic insect introductions in Canada, forest pest-management efforts would benefit from the development of a high-throughput strategy to investigate the phytopathogenic fungal and oomycete species interacting with wood-boring insects. We recycled formerly discarded preservative fluids from the Canadian Food Inspection Agency annual survey using insect traps and analysed more than one hundred samples originating from across Canada. Using the Ion Torrent Personal Genome Machine (PGM) HTS technology and fusion primers, we performed metabarcoding to screen unwanted fungi and oomycetes species, including <i>Phytophthora</i> spp. Community profiling was conducted on the four different wood-boring, insect-attracting semiochemicals; although the preservative (contained ethanol) also attracted other insects. Phytopathogenic fungi (e.g., <i>Leptographium</i> spp<i>.</i> and <i>Meria</i><i>laricis</i> in the pine sawyer semiochemical) and oomycetes (mainly <i>Peronospora</i> spp. and <i>Pythium</i> aff. <i>hypogynum</i> in the General Longhorn semiochemical), solely associated with one of the four types of semiochemicals, were detected. This project demonstrated that the insect traps' semiochemical microbiome represents a new and powerful matrix for screening phytopathogens. Compared to traditional diagnostic techniques, the fluids allowed for a faster and higher throughput assessment of the biodiversity contained within. Additionally, minimal modifications to this approach would allow it to be used in other phytopathology fields.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tremblay</LastName><ForeName>Émilie D</ForeName><Initials>ÉD</Initials><AffiliationInfo><Affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Emilie.Tremblay@canada.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kimoto</LastName><ForeName>Troy</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Canadian Food Inspection Agency, 4321 Still Creek Dr, Burnaby, BC, V5C 6S7, Canada. Troy.Kimoto@canada.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bérubé</LastName><ForeName>Jean A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Natural Resources Canada, Laurentian Forestry Centre, 1055 Du P.E.P.S. Street, P.O. Box 10380 Québec, QC, G1V 4C7, Canada. Jean.Berube@canada.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bilodeau</LastName><ForeName>Guillaume J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, ON, K2H 8P9, Canada. Guillaume.Bilodeau@canada.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>OLF-P-1304, GRDI, QIS and CFIA OFL-P-1411, Genome Canada, and Genome BC (LSARP2112), CFIA-RPS and CFIA-TD and GRDI-CFIA mandated</GrantID><Agency>Canadian Food Inspection Agency</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>J Fungi (Basel)</MedlineTA><NlmUniqueID>101671827</NlmUniqueID><ISSNLinking>2309-608X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">HTS</Keyword><Keyword MajorTopicYN="N">forest</Keyword><Keyword MajorTopicYN="N">fungi</Keyword><Keyword MajorTopicYN="N">insects</Keyword><Keyword MajorTopicYN="N">metagenomics</Keyword><Keyword MajorTopicYN="N">oomycete</Keyword><Keyword MajorTopicYN="N">vectors</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30759800</ArticleId><ArticleId IdType="pii">jof5010015</ArticleId><ArticleId IdType="doi">10.3390/jof5010015</ArticleId><ArticleId IdType="pmc">PMC6463110</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Microbiology. 1999 Apr;145 ( Pt 4):955-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10220175</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2004 Apr;108(Pt 4):411-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15209281</ArticleId></ArticleIdList></Reference><Reference><Citation>Folia Microbiol (Praha). 2004;49(2):99-104</Citation><ArticleIdList><ArticleId IdType="pubmed">15227778</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2005 Jun;166(3):1063-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15869663</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2006 Mar;110(Pt 3):346-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16483756</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2006 Nov;110(Pt 11):1309-22</Citation><ArticleIdList><ArticleId IdType="pubmed">17070028</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 2006 Sep-Oct;98(5):771-80</Citation><ArticleIdList><ArticleId IdType="pubmed">17256580</ArticleId></ArticleIdList></Reference><Reference><Citation>Naturwissenschaften. 2007 Aug;94(8):697-701</Citation><ArticleIdList><ArticleId IdType="pubmed">17429600</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2008 Jul;9(4):403-23</Citation><ArticleIdList><ArticleId IdType="pubmed">18705857</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2008 Aug;101(4):1253-65</Citation><ArticleIdList><ArticleId IdType="pubmed">18767735</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2002 Apr;92(4):393-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18942952</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2002 Sep;92(9):946-55</Citation><ArticleIdList><ArticleId IdType="pubmed">18944019</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2007 Sep;97(9):1119-29</Citation><ArticleIdList><ArticleId IdType="pubmed">18944177</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 1998 Apr;88(4):282-91</Citation><ArticleIdList><ArticleId IdType="pubmed">18944950</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 1998 Jan;88(1):39-44</Citation><ArticleIdList><ArticleId IdType="pubmed">18944997</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 1997 Sep;87(9):888-91</Citation><ArticleIdList><ArticleId IdType="pubmed">18945058</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2009 Apr;99(4):328-38</Citation><ArticleIdList><ArticleId IdType="pubmed">19271973</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2011;49:291-315</Citation><ArticleIdList><ArticleId IdType="pubmed">19400639</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2009 Jul;10(4):537-45</Citation><ArticleIdList><ArticleId IdType="pubmed">19523106</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Entomol. 2010;55:521-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19743916</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2009 Dec;75(23):7537-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19801464</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2010 May;7(5):335-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20383131</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 2010 Jun;36(6):570-83</Citation><ArticleIdList><ArticleId IdType="pubmed">20437083</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 2011 Jan-Feb;103(1):152-63</Citation><ArticleIdList><ArticleId IdType="pubmed">20943533</ArticleId></ArticleIdList></Reference><Reference><Citation>Persoonia. 2010 Dec;25:94-108</Citation><ArticleIdList><ArticleId IdType="pubmed">21339969</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2011 Jul;191(2):314-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21557749</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Bot. 2011 Mar;98(3):426-38</Citation><ArticleIdList><ArticleId IdType="pubmed">21613136</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Biol Sci. 2011 Oct 7;278(1720):2866-73</Citation><ArticleIdList><ArticleId IdType="pubmed">21752822</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2011 Oct;104(5):1592-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22066189</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(12):e28341</Citation><ArticleIdList><ArticleId IdType="pubmed">22174791</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(2):e31410</Citation><ArticleIdList><ArticleId IdType="pubmed">22384018</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2013 Feb 15;29(4):494-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23314324</ArticleId></ArticleIdList></Reference><Reference><Citation>Zookeys. 2013 Jun 13;(309):13-48</Citation><ArticleIdList><ArticleId IdType="pubmed">23794927</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Entomol. 2014;59:13-30</Citation><ArticleIdList><ArticleId IdType="pubmed">24112110</ArticleId></ArticleIdList></Reference><Reference><Citation>Zookeys. 2013 Nov 20;(353):1-24</Citation><ArticleIdList><ArticleId IdType="pubmed">24294095</ArticleId></ArticleIdList></Reference><Reference><Citation>Viruses. 2014 Jan 06;6(1):106-36</Citation><ArticleIdList><ArticleId IdType="pubmed">24399207</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2014 May;17(5):624-36</Citation><ArticleIdList><ArticleId IdType="pubmed">24528408</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2014 Jun;104(6):564-74</Citation><ArticleIdList><ArticleId IdType="pubmed">24548214</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 May 14;9(5):e96611</Citation><ArticleIdList><ArticleId IdType="pubmed">24827724</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2014 Jul;104(7):733-48</Citation><ArticleIdList><ArticleId IdType="pubmed">24915428</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2014 Nov 28;346(6213):1256688</Citation><ArticleIdList><ArticleId IdType="pubmed">25430773</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Mar 31;10(3):e0120571</Citation><ArticleIdList><ArticleId IdType="pubmed">25826684</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2015;53:425-44</Citation><ArticleIdList><ArticleId IdType="pubmed">26047558</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2015 Jun;108(3):1136-47</Citation><ArticleIdList><ArticleId IdType="pubmed">26470239</ArticleId></ArticleIdList></Reference><Reference><Citation>J Econ Entomol. 2015 Aug;108(4):1860-8</Citation><ArticleIdList><ArticleId IdType="pubmed">26470328</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Microbiol. 2017 Mar;199(2):377-383</Citation><ArticleIdList><ArticleId IdType="pubmed">27743015</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 2017 Mar 24;80(3):756-770</Citation><ArticleIdList><ArticleId IdType="pubmed">28199101</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycotoxin Res. 2017 May;33(2):121-127</Citation><ArticleIdList><ArticleId IdType="pubmed">28265970</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2017 Jun;37:54-60</Citation><ArticleIdList><ArticleId IdType="pubmed">28486146</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2017 Sep 15;33(18):2938-2940</Citation><ArticleIdList><ArticleId IdType="pubmed">28645171</ArticleId></ArticleIdList></Reference><Reference><Citation>IMA Fungus. 2017 Jun;8(1):1-15</Citation><ArticleIdList><ArticleId IdType="pubmed">28824836</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2018 Dec;108(12):1509-1521</Citation><ArticleIdList><ArticleId IdType="pubmed">29923801</ArticleId></ArticleIdList></Reference><Reference><Citation>Biology (Basel). 2018 Nov 27;7(4):null</Citation><ArticleIdList><ArticleId IdType="pubmed">30486337</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2016 Jun;100(6):1161-1169</Citation><ArticleIdList><ArticleId IdType="pubmed">30682273</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2017 Jul;101(7):1162-1170</Citation><ArticleIdList><ArticleId IdType="pubmed">30682972</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2012 Aug;96(8):1080-1103</Citation><ArticleIdList><ArticleId IdType="pubmed">30727075</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2009 Jul;93(7):720-726</Citation><ArticleIdList><ArticleId IdType="pubmed">30764365</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Tremblay, Emilie D" sort="Tremblay, Emilie D" uniqKey="Tremblay E" first="Émilie D" last="Tremblay">Émilie D. Tremblay</name></noRegion><name sortKey="Berube, Jean A" sort="Berube, Jean A" uniqKey="Berube J" first="Jean A" last="Bérubé">Jean A. Bérubé</name><name sortKey="Bilodeau, Guillaume J" sort="Bilodeau, Guillaume J" uniqKey="Bilodeau G" first="Guillaume J" last="Bilodeau">Guillaume J. Bilodeau</name><name sortKey="Kimoto, Troy" sort="Kimoto, Troy" uniqKey="Kimoto T" first="Troy" last="Kimoto">Troy Kimoto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000482 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000482 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30759800
|texte= High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30759800" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |